Search results for " expression"
Article
Optimization of Protein Expression in Escherichia Coli
Many problems are associated with protein expression, including toxicity, misfolding and degradation, aggregation in inclusion bodies, low yields, and difficulties in purification. One of the most com…
Article
Platform Technologies Improve Protein Expression
In addition to exploring novel production methods such as continuous processes and disposable production technology, manufacturers also heavily invest in platform technologies for protein expression t…
Article
Cell-free Expression Systems Pose Cell Culture Alternative
One approach that potentially offers the benefit of a “cleaner” biologic product in upstream processing is the use of cell-free expression systems. By producing less waste upstream, there is the poten…
Article
E. coli growth and Dab expression in single-use and stainless steel fermentors
Cell growth,
cell density, and Dab expression levels were assessed and compared between the two production vessels. The results show that the performance of the XDR-50 MO fermentor is comparable …
Article
A Risk-Based Genetic Characterization Strategy for Recombinant CHO Cell Lines Used for Clinical and Commercial Applications
Given this inherent genetic flux, recombinant genes (or transgenes, also referred to as the expression construct) expressed in CHO cells—used for therapeutic bioproduct development—can be subject to g…
Article
Generating a Fully Processed Antibody
This is mainly due to problems getting different levels of protein expression for each of the multiple proteins needed for function and the ability of the system to obtain stable expression of each of…
Article
Fermentation for the Future
Microbial expression is often preferred over cell culture for the production of smaller peptides and proteins that do not require glycosylation because the desired substances may be produced in much s…
Article
Antibody Production in Microbial Hosts
Mammalian cells have been primarily applied as the hosts of choice for mAb expression. However, clinical success of mAbs has tremendously increased their demand, and this in turn has fueled interest i…
Article
Emerging Therapies Test Existing Bioanalytical Methods
Gene therapy, the transfer of genetic material into specific cells to modulate gene expression or produce a new/modified protein, was first tested in a clinical trial in 1990 (1). Researchers at the N…
Article
Best Practices for Selecting a Top-Quality Cell Line
“Equally important,” stresses Fisch, “is the quality of the recombinant DNA expression and transfection systems. Incorporating recombinant DNA stably into a cell line’s genome in a manner that promote…